The NR2F2 gene encodes the transcription factor COUP-TFII, which is upregulated in embryonic mesoderm.Heterozygous variants in NR2F2 cause a spectrum of congenital anomalies including cardiac and gonadal phenotypes.We generated heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2-knockout induced pluripotent garmin 67 stem cell (iPSC) lines from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming.Both iPSC lines exhibited a normal karyotype, typical pluripotent cell morphology, pluripotency marker expression, and the capacity to differentiate into the three pom pom cupcake toppers embryonic germ layers.These lines will allow us to explore the role of NR2F2 during development and disease.